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Nucleic Acid Technology : Focus on DNA and RNA Applications

par: Dr A.Galleze , Dr F.Djaballah-Ider & Dr I.Gouaref
Disponible
Pages 116
Dimensions 16*23.5
Publication 2025-12-31
Éditeur —
Papier : 950.00 DA Numérique: 475.00 DA

The field of nucleic acid technology has undergone a remarkable transformation over the past few decades, evolving from fundamental discoveries in nucleic acids structure to powerful tools that underpin modern biotechnology, diagnostics, and therapeutics. This book, Nucleic Acid Technology, is designed to provide a comprehensive overview of the principles, methodologies, and applications that define this rapidly advancing discipline. It aims to bridge the gap between foundational concepts and cutting-edge innovations, offering readers both theoretical insights and practical knowledge. Topics span from the chemistry and structure of nucleic acids to advanced techniques such as PCR, sequencing, CRISPR-based editing, nucleic acid therapeutics, and nanotechnology applications. The text is intended for students, researchers, and professionals in molecular biology, biotechnology, genetics, and related fields. Each chapter integrates historical perspectives, current developments, and future directions, encouraging readers to appreciate not only the technical depth but also the societal and ethical implications of nucleic acid technologies. We express our gratitude to the many scientists whose pioneering work has laid the foundation for this field and to the contributors and reviewers who helped refine the content of this book. It is our hope that this volume will serve as both a learning resource and an inspiration for future explorations in the molecular sciences.

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Preface 03
Table of Contents 05
Chapter 1: Nucleic acid probes and its applications 07
1. Definition 08
2. Nucleic probe labelling 09
3. Application of nucleic acid probe in analysis and detection 12
Exercises 16
Solutions 18
Chapter 2: PCR and Its Variations 23
1. Definition 24
2. The PCR Cycle 25
3. PCR Variations 26
4. Polymerase chain reaction applications 35
Exercises 38
Solutions 40
Chapter 3: Molecular Cloning 43
1. Definition 44
2. Tools of molecular cloning 44
3. The cloning technique 48
4. Applications of molecular cloning 49
Exercises 51
Solutions
Chapter 4: Transfection and sequencing 53
1. Definition 54
2. Physical methods 56
3. Biological methods 60
4. Chemical transfection 65
5. Biomedical applications of CRISPR/Cas9 system 75
Exercises 78
Solutions 79
Chapter 5: Sequencing 81
1. Definition 82
2. Sequencing technologies 82
3. Sequencing applications in Research and Diagnostics 90
Exercises 98
Solutions 99
Chapter 6: Annexes 101
Glossary 102
Bibliography
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